91探花 the PPS Team
Here you can find contact information to our team and a list of the expert areas we are responsible of.
| STEP OF THE PROCESS | DESCRIPTION | WHO TO CONTACT FOR INITIAL DISCUSSION |
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Expression vector design |
Design of expression vector (plasmid) for use in protein expression below. |
All, depending on expression host |
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Protein expression host: |
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Bacteria |
Protein expression in various E. coli hosts |
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Yeast |
Protein expression in the yeast Pichia pastoris |
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Insect cells |
Protein expression in Sf9 or HighFive insect cells using the baculovirus expression vector system (BEVS). |
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Plant cells |
Protein expression in plant cells by infection with Agrobacterium tumefaciens |
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Mammalian cells |
Protein expression in CHO or HEK293 cells or other cell lines |
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Cell free protein extracts |
In Cell-free protein synthesis the translation machinery is isolated and protein synthesis is carried out from a plasmid template in vitro |
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Purification |
Protein purification using various chromatographic techniques as for example IMAC, IEX, SEC |
All |
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Speciality - Gateway Modules: |
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(Per)deuteration |
Biological (per)deuteration of proteins and development/feasibility studies for this purpose |
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Molecular chaperones |
Production of antibody fragments for co-crystallization purposes (can include production of the antigen) |
Kristina Hedfalk
